Publications: Metabolism & Microbiome
Geoff's dog, Patton (RIP), eating Hill's Pet Science Diet to stay nourished and active while backpacking in Wyoming's Bridger-Teton wilderness, August 2014
"Food as medicine" polyphenol-rich plant fibers impart health benefits in companion animals and people! In this clinical canine study, we used metabolomics and microbiome sequencing to show how a novel prebiotic fiber bundle uniquely improves pathway markers of gastrointestinal and systemic host health, particularly through promoting saccharolytic and suppressing putrefactive microbial metabolism, while generating beneficial fecal postbiotics and circulating anti-inflammatory signatures.
Chronic large-bowel diarrhea is a common occurrence in pet dogs. While nutritional intervention is considered the primary therapy, the metabolic and gut microfloral effects of fiber and polyphenol-enriched therapeutic foods are poorly understood.
This prospective clinical study enrolled 31 adult dogs with chronic, active large bowel diarrhea from private veterinary practices. Enrolled dogs received a complete and balanced dry therapeutic food containing a proprietary fiber bundle for 56 days. Metagenomic and metabolomic profiling were performed on fecal samples at Days 1, 2, 3, 14, 28, and 56; metabolomic analysis was conducted on serum samples taken at Days 1, 2, 3, 28, and 56.
In dogs with chronic diarrhea, feeding specific dietary fibers increased gut saccharolysis and bioavailable phenolic and indole-related compounds while suppressing putrefaction. These changes were associated with improved markers of gut inflammation and stool quality.
Part of the efficacy of the gut microbiome "activation" described in this video for Activbiome+ prebiotic in companion animals is a shift away from undesirable proteolytic bacterial activity towards more beneficial saccharolytic metabolism, as described in these two manuscripts.
White Papers
Immunotherapy, sample prep, and biospecimens
Clinical trials usually proceed in a prospective manner, where specimens and data are gathered as the trial unfolds. Thanks to Sanguine's direct-to-patient approach, researchers can prospectively design discovery, translational, and supplemental clinical studies and obtain annotated human biospecimens from patients and healthy donors just as you would a clinical trial.
This white paper was designed to read like a comprehensive review describing how the prospective collection of biospecimens aligns with precision medicine principles.
Scientists from Charles River Laboratories and Curiox Biosystems collaborated to characterize the performance and workflow of Laminar WashTM systems in isolating and preparing tumor-infiltrating leukocytes (TILs) from anti-PD1-challenged CT26 mice as a model system.
This white paper was written in the style of a research article to showcase the utility of the Laminar Wash technology in a high-value application, immunotherapy research sample preparation, and provide potential customers with a flow cytometry workflow example.
High quality data begins with high quality sample preparation. Designed to encourage researchers conducting single-cell genomics experiments, this white paper aims to convince sample prep reproducibility and automation should be considered in obtaining the highest quality data, using published and webinar-presented examples.
Before starting Geocyte, Geoff worked at Curiox. He conceptualized, outlined, instructed the primary literature material, generated the graphics, and edited this white paper, which was written by a contractor through Fierce Biotech.
Publications: Structural Biology
Read about Geoff's publications from when he was a biophysical chemistry student and postdoc at our Structural Biology Applications page, including:
- Anthrax toxin and protein translocation structure and function
- Biology with X-ray lasers (i.e., blowing up tiny crystals with X-ray lasers, pew pew pew!)
- Virulence factors from the biothreat agent Francisella tularensis
